abstract：:Nasal metabolism of inhaled material may influence its biological fate and toxicity. The purpose of this study was to investigate, in a noninvasive and qualitative manner, the in vivo nasal metabolic activity towards 1-(3,4-methylenedioxyphenyl)propane (dihydrosafrole). Dihydrosafrole was the compound of choice as a r...

journal_title：Chemico-biological interactions

authors： Petridou-Fischer J,Whaley SL,Dahl AR

更新日期：1987-01-01 00:00:00

abstract：:In isolated frog heart it was established that methylene-blue (MB, an oxidizing agent) decreased, while ascorbate (ASC, a reducing agent) increased the frequency of autorhythmic heart contractions. After MB treatment, in parallel with this phenomenon, the extracellular K+ concentration [K+]o showed a slow increase, bu...

journal_title：Chemico-biological interactions

authors： Wittmann I,Puppi A,Dely M

更新日期：1987-01-01 00:00:00

abstract：:Structure-uncoupling activity relationship of seven anthraquinone derivatives were investigated using rat liver mitochondria. Three compounds bearing the free hydroxyl group at the beta-position of their anthraquinone nucleus (1,3,6,8-tetrahydroxyanthraquinone, 1-acetyl-2,4,5,7-tetrahydroxy-9,10-anthracenedione and sk...

journal_title：Chemico-biological interactions

authors： Betina V,Kuzela S

更新日期：1987-01-01 00:00:00

abstract：:The peroxyl radicals CF3O2., CCl3O2. and CBr3O2. were produced by radiolysis of aerated aqueous-alcohol solutions of CF3Br, CF3Cl, CCl4 or CBr4. Kinetic spectrophotometric pulse radiolysis experiments were carried out in the presence of various substrates: urate, ascorbate, xanthine, hydroquinone, p-methoxyphenol, phe...

journal_title：Chemico-biological interactions

authors： Huie RE,Brault D,Neta P

更新日期：1987-01-01 00:00:00

abstract：:Styrene oxide was reacted with deoxynucleosides and DNA in aqueous buffer at pH 7.4. The products were purified by HPLC, characterized by UV spectroscopy and by chemical ionization mass spectrometry. The main products identified were 7-alkyl-, N2-alkyl- and O6-alkyldeoxyguanosine, 1-alkyl-, and N6-alkyldeoxyadenosine,...

journal_title：Chemico-biological interactions

authors： Savela K,Hesso A,Hemminki K

更新日期：1986-12-01 00:00:00

abstract：:Alkyl adducts at the O6-position of guanine constitute promutagenic DNA lesions likely to be involved in the initiation of malignant transformation. They can be removed by a cellular acceptor protein termed O6-alkylguanine-DNA alkyltransferase (AT). In rat liver this repair enzyme can be induced by a variety of hepato...

journal_title：Chemico-biological interactions

authors： Schmerold I,Spath A

更新日期：1986-12-01 00:00:00

abstract：:The microsomal metabolism of pentachlorophenol (PCP) was investigated, with special attention to the conversion dependent covalent binding to protein and DNA. The two metabolites detected were tetrachloro-1,2- and tetrachloro-1,4-hydroquinone. Microsomes from isosafrole (ISF)-induced rats were by far the most effectiv...

journal_title：Chemico-biological interactions

authors： van Ommen B,Adang A,Müller F,van Bladeren PJ

更新日期：1986-10-15 00:00:00

abstract：:The feasibility of an oxygen-independent mechanism of chloroform bioactivation was indicated by the covalent binding to lipid and protein occurring in anaerobic incubations of CHCl3 and microsomes in the presence of NADPH. Under these conditions, the loss of cytochrome P-450 and the inhibition of related monoxygenases...

journal_title：Chemico-biological interactions

authors： Testai E,Vittozzi L

更新日期：1986-09-01 00:00:00

abstract：:A single intravenous injection of methylazoxymethanol (MAM) acetate in doses of either 20 or 35 mg/kg body weight to male Sprague-Dawley rats induced altered liver cell foci and later, liver neoplasms in a dose related manner. Sequential observations in the rats given 35 mg/kg and thereafter fed an iron-loading diet r...

journal_title：Chemico-biological interactions

authors： Tanaka T,Mori H,Hirota N,Furuya K,Williams GM

更新日期：1986-04-01 00:00:00

abstract：:This study examines the relationship between cell progress into the state of unbalanced growth, hydrolytic enzyme activities and cell survival during the exposure of L5178Y cells to hydroxyurea (HU), excess thymidine (dThR), hydroxyurea with excess of four deoxyribonucleosides (dNR) or excess dTHR with deoxycytidine (...

journal_title：Chemico-biological interactions

authors： Malec J,Przybyszewski WM,Grabarczyk M,Sitarska E,Czartoryska B

更新日期：1986-03-01 00:00:00

abstract：:Since endogenous glutathione (GSH), the main non-protein intracellular thiol compound, is known to provide protection against reactive radical species, its depletion by diethylmaleate (DEM) was used to assess the role of free radical formation mediated by doxorubicin in DNA damage, cytotoxicity and mutagenicity of the...

journal_title：Chemico-biological interactions

authors： Capranico G,Babudri N,Casciarri G,Dolzani L,Gambetta RA,Longoni E,Pani B,Soranzo C,Zunino F

更新日期：1986-02-01 00:00:00

abstract：:Examination of the high pressure liquid chromatographic profiles of ethyl acetate extractable benzo [a] pyrene (B(a)P)-metabolites from human lung fibroblast and type II epithelial cells after S phase entry indicated that B(a)P-7,8-diol and 9,10-diol species were produced following the oxygenation of B(a)P. These meta...

journal_title：Chemico-biological interactions

authors： Milo GE

更新日期：1985-12-31 00:00:00

abstract：:The metabolic activation of [14C]phenol resulting in covalent binding to proteins has been studied in rat liver microsomes. The covalent binding was dependent on microsomal enzymes and NADPH and showed saturation kinetics for phenol with a Km-value of 0.04 mM. The metabolites hydroquinone and catechol were formed at r...

journal_title：Chemico-biological interactions

authors： Wallin H,Melin P,Schelin C,Jergil B

更新日期：1985-11-01 00:00:00

abstract：:The secretion of digoxin and digitoxin into in situ perfused jejunal and colonic segments of normal or quinidine treated guinea pigs was studied. Quinidine was administered intravenously by constant rate infusion resulting in a quinidine plasma concentration of about 6 micrograms/ml. After 2 h digoxin or digitoxin was...

journal_title：Chemico-biological interactions

authors： Schäfer SG,Schuhmann G,Doering W,Fichtl B

更新日期：1985-10-01 00:00:00

abstract：:The carcinogenic activity of the synthetic estrogen hexestrol was measured in male Syrian hamsters. Between 90% and 100% of the animals treated with hexestrol or with 3',3",5',5"-tetradeuteriohexestrol, implanted subcutaneously as 25-mg pellets, were found with renal carcinoma after 6-7 months. In vitro hexestrol meta...

journal_title：Chemico-biological interactions

authors： Liehr JG,Ballatore AM,Dague BB,Ulubelen AA

更新日期：1985-10-01 00:00:00

abstract：:The administration of the placental hormone human chorionic gonadotrophin (HCG) to 50-day-old virgin Sprague--Dawley rats has been shown to reduce the incidence of 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary cancer. We now report from studies using rat mammary epithelial cells in culture that the anti-carcin...

journal_title：Chemico-biological interactions

authors： Tay LK,Russo J

更新日期：1985-10-01 00:00:00

abstract：:The toxicity of halogenated alkanes originates from their metabolism by cytochrome P-450 which leads to the formation of reactive intermediates. In particular, peroxyl radicals derived from the halogenated compounds are believed to induce peroxidative chain degradation of lipids. To examine this hypothesis, radical re...

journal_title：Chemico-biological interactions

authors： Brault D,Neta P,Patterson LK

更新日期：1985-08-01 00:00:00

abstract：:Using a rat liver cytosol source of enzyme trialkyl phosphorothioates have been shown to be substrates of glutathione S-transferases. Using OSS-trimethyl phosphorodithioate (OSS-Me(O] and OOS-trimethyl phosphorothioate (OOS-Me(O] the methyl transferred to the sulphydryl of glutathione is that attached to phosphorus vi...

journal_title：Chemico-biological interactions

authors： Aldridge WN,Grasdalen H,Aarstad K,Street BW,Norkov T

更新日期：1985-07-01 00:00:00

abstract：:Trans-4-acetylaminostilbene (trans-AAS) is a potent carcinogen and quite specifically produces sebaceous gland tumors, predominantly in the Zymbal's gland of rats. It is also acutely toxic to the rat glandular stomach. Recent results have shown that these target tissues are not notably exposed to reactive metabolites ...

journal_title：Chemico-biological interactions

authors： Hilpert D,Neumann HG

更新日期：1985-06-01 00:00:00

abstract：:Pyrethroid interactions with dipalmitoyl phosphatidylcholine (DPPC) vesicles have been characterized in bilayers having large and small radii of curvature. The abilities of pyrethroids to alter the gel-fluid phase transition profiles were determined by steady state fluorescence anisotropy and phase-modulation lifetime...

journal_title：Chemico-biological interactions

authors： Stelzer KJ,Gordon MA

更新日期：1985-06-01 00:00:00

abstract：:The structural features which determine interaction of safrole and related methylenedioxyphenyl compounds with cytochromes P-450 or P-448, and determine the induction of these two classes of the cytochrome, have been studied. All methylenedioxyphenyl compounds studied interact with both cytochromes P-450 and P-448 eli...

journal_title：Chemico-biological interactions

authors： Ioannides C,Delaforge M,Parke DV

更新日期：1985-05-01 00:00:00

abstract：:The structure of the covalent adduct formed in vitro between [14C]-acetaminophen ([14C]APAP) and bovine serum albumin (BSA) has been investigated with the aid of new analytical methodology. The APAP-BSA adduct, isolated from mouse liver microsomal incubations to which the radiolabeled drug and BSA had been added, was ...

journal_title：Chemico-biological interactions

authors： Hoffmann KJ,Streeter AJ,Axworthy DB,Baillie TA

更新日期：1985-02-01 00:00:00

abstract：:Addition of the tumor promoter phorbol myristate acetate to polymorphonuclear leukocytes results in the oxidation of the arylamine carcinogens; [14C]benzidine, N-[14C]methylaminoazobenzene and [14C]aminofluorene to reactive intermediate(s) that bind irreversibly to the leukocyte DNA. The binding was dependent on oxyge...

journal_title：Chemico-biological interactions

authors： Tsuruta Y,Subrahmanyam VV,Marshall W,O'Brien PJ

更新日期：1985-02-01 00:00:00

abstract：:Colon epithelium is made up of two general classes of cells, surface cells which are post-mitotic and crypt cells which contain the proliferative population. Their relative vulnerability to environmental damage and ability to perform DNA repair are important issues in colon carcinogenesis. DNA damage and repair was st...

journal_title：Chemico-biological interactions

authors： Kulkarni MS,Yielding KL

更新日期：1985-01-01 00:00:00

abstract：:Intraperitoneal administration of chloroquine, primaquine and quinacrine to rats resulted in inhibition of the hepatic microsomal mixed-function oxidases. The N-demethylation of benzphetamine (cytochrome P-450) was inhibited by chloroquine only while the O-deethylation of ethoxyresorufin (cytochrome P-448) was inhibit...

journal_title：Chemico-biological interactions

authors： Thabrew MI,Ioannides C

更新日期：1984-10-01 00:00:00

abstract：:The FAD-containing monooxygenase (FMO) has been purified from both mouse and pig liver microsomes by similar purification procedures. Characterization of the enzyme from these two sources has revealed significant differences in catalytic and immunological properties. The pH optimum of mouse FMO is slightly higher than...

journal_title：Chemico-biological interactions

authors： Sabourin PJ,Hodgson E

更新日期：1984-09-15 00:00:00

abstract：:Unscheduled DNA synthesis (UDS), following exposure to dimethylnitrosamine (DMN), was potentiated in cultured hepatocytes isolated following treatment of rats for 14 or 28 days with 20% ethanol/5% sucrose solution. Ethanol treatment was associated with increased UDS, a concomitant increase in hepatic microsomal protei...

journal_title：Chemico-biological interactions

authors： Olson MJ,Pounds JG,Casciano DA

更新日期：1984-08-01 00:00:00

abstract：:This paper reports on time-resolved microfluorimetric measurements on hematoporphyrin-derivative (HpD)-treated lymphocytes. HpD is at present widely used as a tumor-locating and photosensitizing drug. It is therefore of great importance to study the extent to which the HpD uptake process depends on cell functional and...

journal_title：Chemico-biological interactions

authors： Docchio F,Ramponi R,Sacchi CA,Bottiroli G,Freitas I

更新日期：1984-07-01 00:00:00

abstract：:Variable temperature 1H-nuclear magnetic resonance (NMR) has been used to study the interaction of the RNA trimer, GpCpA, with the intercalators ethidium bromide and the acridine derivatives; proflavin, 9-amino-acridine, acridine orange, acridine yellow and acriflavin. The complexes formed were studied at nucleic acid...

journal_title：Chemico-biological interactions

authors： Coddington JM,Alkema D,Bell RA,Hughes DW,Neilson T

更新日期：1984-06-01 00:00:00

abstract：:Plasma protein S-sulfonate compounds (RS-SO-3) have previously been shown to form, presumably by sulfitolysis of disulfide bonds, as a result of exposure to sulfite. In the investigations reported here, we identify two proteins in rabbit plasma, namely albumin and plasma fibronectin, which contain reactive sites for S...

journal_title：Chemico-biological interactions

authors： Gregory RE,Gunnison AF

更新日期：1984-04-01 00:00:00

abstract：:The metabolism and genotoxicity of 1,2-dibromoethane (EDB) and its deuterium substituted analog ( d4EDB ) were studied in isolated rat hepatocytes. There was a marked isotope effect on the metabolism of EDB by hepatocytes. This was due to decreased microsomal oxidation of d4EDB . Cytosolic metabolism of EDB, as measur...

journal_title：Chemico-biological interactions

authors： White RD,Petry TW,Sipes IG

更新日期：1984-04-01 00:00:00

abstract：:Rabbit bone marrow mitochondria, stripped of their outer membrane (mitoplasts), have been shown to carry out the NADPH-dependent bioactivation of radiolabelled benzene in vitro to metabolites capable of covalently binding to mtDNA, thereby inhibiting transcription. The metabolites of benzene produced in bone marrow ce...

journal_title：Chemico-biological interactions

authors： Rushmore T,Snyder R,Kalf G

更新日期：1984-04-01 00:00:00

abstract：:S1 nuclease hydrolysis and benzoylated naphthoylated DEAE cellulose (BND-cellulose) chromatography have been used to study the effect of riboflavin and visible light on DNA. Native calf thymus DNA was incubated with riboflavin in the presence of fluorescent light for various time periods and subjected to S1 nuclease h...

journal_title：Chemico-biological interactions

authors： Alvi NK,Ahmad NS,Ahmad S,Hadi SM

更新日期：1984-03-01 00:00:00

abstract：:At pH 7.35, N-(2-oxopropyl)-N-nitrosourea (OPNU) reacted with calf thymus DNA to yield O6-methylguanine, 7-methylguanine and 3-methyladenine. Kinetic measurements of the base catalyzed decomposition of OPNU and the extent of methylation of DNA by OPNU suggested that methylnitrosourea is not formed as an intermediate p...

journal_title：Chemico-biological interactions

authors： Leung KH,Archer MC

更新日期：1984-02-01 00:00:00

abstract：:Butylated hydroxytoluene (BHT) at concentrations of 300-6000 ppm in the diet caused a dose-dependent increase in gamma-glutamyl transpeptidase (GGT) activity in normal F344 male rat liver at 18 weeks. However, the activities of glutathione S-transferases (GSTs) of rat liver cytosol were enhanced only at concentrations...

journal_title：Chemico-biological interactions

authors： Furukawa K,Maeura Y,Furukawa NT,Williams GM

更新日期：1984-01-01 00:00:00

abstract：:We have directly compared in resting human mononuclear leukocytes the DNA repair effects caused by ADP-ribosyl transferase (ADPRT) activity following DNA damage induction by gamma radiation, UV radiation, ethylene oxide (EO) and N-acetoxy-2-acetylaminofluorene (NA-AAF). The presence of inhibitors of ADPRT during the q...

journal_title：Chemico-biological interactions

authors： Pero RW,Jonsson GG,Persson L

更新日期：1983-12-01 00:00:00

abstract：:The ochratoxin A (OTA) metabolite (4R)-4-hydroxyochratoxin A [4R)-OTA) inhibits the aminoacylation of phenylalanine tRNA catalyzed by phenylalanyl-tRNA synthetase (PheRS) with a Ki-value of 0.9 mM as compared to 1.3 mM for OTA. It also inhibits protein synthesis and cell growth in the same manner as OTA. Ochratoxin al...

journal_title：Chemico-biological interactions

authors： Creppy EE,Størmer FC,Kern D,Röschenthaler R,Dirheimer G

更新日期：1983-11-01 00:00:00

abstract：:Product yields have been determined after one-electron-induced reduction of CCl4 in aqueous solutions containing t-butanol and various concentrations of oxygen. It was shown that CCl3 radicals add oxygen to form CCl3O2 radicals, which eventually yield three chloride ions and CO2. A constant ratio of G(Cl-)/G(CO2) = 4 ...

journal_title：Chemico-biological interactions

authors： Mönig J,Bahnemann D,Asmus KD

更新日期：1983-10-15 00:00:00

abstract：:The distribution of 203Hg after inhalation of radioactive metallic mercury (4.3 mumol 203Hg0/kg body wt. for 1 h) was studied in saline (control) and selenite (10 mumol/kg body wt. intraperitoneally, i.p.) pretreated male C57BL mice by means of whole-body autoradiography and scintillation counting. Selenite pretreatme...

journal_title：Chemico-biological interactions

authors： Khayat A,Dencker L

更新日期：1983-09-15 00:00:00

abstract：:Alkylation of DNA with N-methyl-N-nitrosourea (MeNU) and N-ethyl-N-nitrosourea (EtNU) reduces its ability to support RNA synthesis catalyzed by exogenously added RNA polymerase. It is likely that 7-alkylguanine and alkyl phosphotriester in DNA are mainly responsible for the inhibition of RNA synthesis. The inhibitory ...

journal_title：Chemico-biological interactions

authors： Marushige K,Marushige Y

更新日期：1983-09-01 00:00:00